Characterization of Synthetic Hydroxyproline-Rich Proteoglycans with Arabinogalactan Protein and Extensin Motifs in Arabidopsis
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چکیده
منابع مشابه
Characterization of synthetic hydroxyproline-rich proteoglycans with arabinogalactan protein and extensin motifs in Arabidopsis.
A series of gene constructs encoding synthetic glycomodule peptides with N-terminal signal sequences and C-terminal green fluorescent proteins were expressed in transgenic Arabidopsis (Arabidopsis thaliana) under the control of the 35S promoter. The synthetic glycomodule peptides were composed of repetitive proline-containing motifs that have been previously found to be substrates for prolyl hy...
متن کاملCharacterization of synthetic hydroxyproline-rich proteoglycans with AGP- and extensin- motifs in Arabidopsis
Abbreviations. AGP, arabinogalactan protein; EGFP, enhanced green fluorescent protein; GPI, glycosylphosphatidylinositol; GAGP, gum arabic glycoprotein; GT, glycosyltransferase; HRGP, hydroxyproline rich-glycoprotein; LeAGP1, arabinogalactan protein 1 from tomato; Lys, lysine rich domain; P4H, prolyl-4-hydroxylase; (SP), serine-proline repeat; (VP), valine-proline repeats; (TP), threonine-proli...
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AtAGP17, AtAGP18 and AtAGP19 are homologous genes encoding three putative glycosylphosphatidylinositol (GPI)-anchored classical arabinogalactan-proteins (AGPs) in Arabidopsis. They are distinguished from other AGPs by a short, C-terminal lysine-rich region. Organ-specific expression of these genes was revealed by Northern blot analysis. AtAGP17 was strongly expressed in leaves and stems, and we...
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Hydroxyproline-O-galactosyltransferase (GALT) initiates O-glycosylation of arabinogalactan-proteins (AGPs). We previously characterized GALT2 (At4g21060), and now report on functional characterization of GALT5 (At1g74800). GALT5 was identified using heterologous expression in Pichia and an in vitro GALT assay. Product characterization showed GALT5 specifically adds galactose to hydroxyproline i...
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Recent research, mostly in Arabidopsis thaliana, has led to the identification and characterization of the glycosyltransferases responsible for the biosynthesis of two of the most functionally important and abundant families of plant cell wall proteins, extensins, and arabinogalactan-proteins. Extensin glycosylation involves monogalactosylation of serine residues by O-α-serine galactosyltransfe...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2006
ISSN: 1532-2548
DOI: 10.1104/pp.106.084244